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High EYFP fluorescence is also observed in the ventral gray horn in transverse section of the spinal cord.
The donating investigator also reports that these Ch AT-mh Ch R2-YFP line 6 mice exhibit a similar expression pattern as Ch AT-mh Ch R2-YFP line 5 (JAX Stock No.
This optogenetic strain is one of many from the same transgene creator/donating investigator with light-inducible neurobiology applications; including Thy1-Ch R2-YFP line 18 (Stock No. EYFP expression in cholinergic neurons of the striatum, trochlear nucleus, medial habenula, interpeduncular nucleus and various brainstem motor neuron nuclei.
Ch AT-Ch R2-EYFP line 6 mice also consume more food and water during the dark cycle compared to control mice. There are approximately 50 copies of the transgene present.
Compared to wildtype animals, Ch AT-Ch R2-EYFP line 6 mice show no changes in metabolic profile, locomotor activity in a novel environment, anxiety-like behavior, depression-like behavior, gross sensorimotor function, and ability to use cues to learn a task. Of note, the donating investigator may not have fixed the Y chromosome to the C57BL/6J genetic background during backcrossing.
The mh Ch R2:: YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mh Ch R2; also called h Ch R2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP).
The mh Ch R2 is designed to cause larger stationary photocurrents compared to Ch R2.
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The information presented at this site should not be construed to be formal legal advice nor the formation of a lawyer/client relationship.VACh T overexpression results in increased cholinergic tone; the functional consequence of which is increased physical endurance but severe cognitive deficits in attention, working memory and spatial memory.Ch AT-Ch R2-YFP BAC transgenic mice have expression of the mh Ch R2:: YFP fusion protein directed to cholinergic neuronal populations by the mouse choline acetyltransferase (Chat or Ch AT) promoter/enhancer regions on the BAC transgene.VACh T protein is overexpressed in the hippocampus and brainstem.EYFP expression is visible by direct fluorescence (epifluorescence microscope).The total number of animals provided, their gender and genotype will vary.Tags: Adult Dating, affair dating, sex dating